Principal/Senior Scientist, mRNA engineering

What you'll do As a member of our team, you will: - Execute and scale end-to-end mRNA production, including template preparation, in vitro transcription (IVT), and purification (TFF, chromatography). - Implement rigorous quality control assays, including direct RNA sequencing, fragment analysis, dsRNA quantitation, and residual contaminant analysis. - Author and maintain detailed SOPs and batch records, ensuring all production runs meet GLP standards. - Independently design and execute experiments to improve mRNA sequence potency, specificity, and manufacturing performance. - Train, manage, and mentor other team members on mRNA optimization and production workflows.

Requirements - PhD in molecular biology, biochemistry, cell biology, or a related field or equivalent industry experience (5+ years). - Deep hands-on experience with IVT and the specific challenges of scaling nucleic acid production. - Proficiency with TFF and chromatography systems for the purification of large scale RNA batches. - Ability to perform and interpret mRNA QC assays and troubleshoot mRNA production challenges. - Strong experience in drafting technical reports and standardized protocols.

Nice to have - Familiarity with Python or R for sequence analysis and secondary structure prediction. - Understanding of how mRNA quality attributes impact Lipid Nanoparticle (LNP) formulation and delivery. - Experience with complex plasmid design and bacterial scale-up for DNA template generation. - Experience managing external contract manufacturing organizations (CMOs)

Benefits NewLimit offers generous benefits to ensure our team can focus on our mission: - Health, dental, and vision insurance - 401(k) with company match - Paid parental leave - Industry-leading paid time off (PTO) - 20 days/year for all employees + holidays - Meals and snacks on-site Salary Range: $170,000 to $200,000.

Compensation for the role will depend on a number of factors, including a candidate's qualifications, skills, competencies, and experience. NewLimit offers a competitive benefits package, which includes a 401k, healthcare coverage, and 20 days of PTO. Learn more at: https://www.newlimit.com/careers *This compensation and benefits information is based on Company's good faith estimate as of the date of publication and may be modified in the future.