At Vertex, we invest in scientific innovation to create transformative medicines for people with serious diseases. Our growing Molecular Profiling team is seeking a Senior Scientist, Flow Cytometry to provide deep technical expertise in flow cytometry and cellular profiling to support discovery, translational, and development programs.
This role will lead and drive the generation of high-dimensional insights into cell phenotype, function, and biology that help inform mechanism of action, biomarker strategies, patient stratification, and program decision-making.
The successful candidate will independently own cytometry workstreams across multiple programs and contribute to the scientific direction of molecular profiling efforts.
The ideal candidate is a highly motivated scientist with strong experience in multiparameter and high-dimensional flow cytometry (spectral flow), cell sorting, and immunophenotyping and a proven ability to thrive in a collaborative, fast-paced research environment.
- Design, develop, optimize, and execute flow cytometry-based assays to support research and translational objectives across discovery and development programs
- Lead multicolor immunophenotyping panel design, including marker selection, fluorochrome strategy, controls, and advanced gating and data analysis approaches
- Apply flow cytometry in conjunction with molecular profiling data to enable deeper characterization of cell states, pathway activity, target engagement, and disease-relevant biology
- Partner with cross-functional teams to integrate cellular and molecular datasets, including transcriptomic, proteomic, and biomarker data, to support hypothesis generation and program strategy
- Analyze and interpret complex flow cytometry data to support actionable biological insights, mechanism of action studies, and translational hypotheses
- Establish and apply best practices for sample preparation, staining, acquisition, compensation, quality control, and data analysis
- Troubleshoot technical issues and drive continuous improvement in assay robustness, reproducibility, and efficiency
- Contribute to experimental design, study planning, and strategic program discussions and decision-making
- Prepare technical documentation, protocols, study summaries, and presentations
- Evaluate and implement emerging cytometry and profiling technologies, reagents, and workflows where appropriate
- Mentor junior team members and serve as a scientific resource within the broader organization
- Independently lead cytometry workstreams across multiple programs, ensuring alignment with program goals and timelines
- Influence program decisions through data-driven insights and clear communication of complex biological findings
- Deep expertise in flow cytometry principles, instrumentation, and assay development
- Strong understanding of immune profiling, cellular phenotyping, and molecular profiling strategies
- Experience connecting cell-based phenotypic data with broader molecular signatures and translational biomarkers
- Experience designing and optimizing high-parameter or multicolor flow cytometry panels, including intracellular and phospho-flow analysis
- Proficiency in flow cytometry analysis software such as FlowJo, Dotmatics or equivalent
- Working knowledge of data analysis approaches and familiarity with R or Python-based workflows (preferred)
- Strong scientific reasoning, data interpretation, and problem-solving skills
- Ability to communicate complex technical findings clearly to technical and cross-functional stakeholders
- Strong organizational skills and the ability to manage multiple priorities in a matrixed environment
- Demonstrated ability to operate with a high degree of independence while contributing to team-based goals
- PhD in Immunology, Cell Biology, Molecular Biology, Biology, Biomedical Sciences, or related discipline with 2+ years of relevant experience; or
- Master’s degree with 6+ years of relevant experience; or
- Bachelor’s degree with 8+ years of relevant experience
- Experience with primary human samples, including blood- or tissue-derived specimens
- Familiarity with spectral flow cytometry and cell sorting (BD, Cytek, Sony platforms)
- Experience working in environments where molecular profiling and cell-based assays are jointly used to answer translational questions
- Experience in immunology and inflammation disease areas
- Experience supporting translational research, biomarker strategies, or clinical development
- Experience integrating cytometry data with single-cell genomics, spatial transcriptomics, or proteomics datasets
In this On-Site designated role, you will work five days per week on-site with ad hoc flexibility.