Principal/Senior Scientist, mRNA engineering

- Execute and scale end-to-end mRNA production, including template preparation, in vitro transcription (IVT), and purification (TFF, chromatography).

- Implement rigorous quality control assays, including direct RNA sequencing, fragment analysis, dsRNA quantitation, and residual contaminant analysis.

- Author and maintain detailed SOPs and batch records, ensuring all production runs meet GLP standards.

- Independently design and execute experiments to improve mRNA sequence potency, specificity, and manufacturing performance.

- Train, manage, and mentor other team members on mRNA optimization and production workflows.

- PhD in molecular biology, biochemistry, cell biology, or a related field or equivalent industry experience (5+ years).

- Deep hands-on experience with IVT and the specific challenges of scaling nucleic acid production.

- Proficiency with TFF and chromatography systems for the purification of large scale RNA batches.

- Ability to perform and interpret mRNA QC assays and troubleshoot mRNA production challenges.

- Strong experience in drafting technical reports and standardized protocols.

- Familiarity with Python or R for sequence analysis and secondary structure prediction.

- Understanding of how mRNA quality attributes impact Lipid Nanoparticle (LNP) formulation and delivery.

- Experience with complex plasmid design and bacterial scale-up for DNA template generation.

- Experience managing external contract manufacturing organizations (CMOs)

Salary Range: $170,000 to $200,000.

Compensation for the role will depend on a number of factors, including a candidate's qualifications, skills, competencies, and experience. NewLimit offers a competitive benefits package, which includes a 401k, healthcare coverage, and 20 days of PTO. Learn more at: https://www.newlimit.com/careers *This compensation and benefits information is based on Company's good faith estimate as of the date of publication and may be modified in the future.